How to take GHRP-6?

[LuvMuhRoids]

I have a 1mg/ml bottle here and for the life of me I cant figure out how to take it. I spent an hour surfing the net and even AR and there is basically nothing. Anyone here know how to administer this stuff. I thought it was injectable to but Im reading it is SC taken?

[LuvMuhRoids]

Are there any recommended procedures when I am about to start working on my peptide?


When preparing the peptide for use, please observe the following steps to maintain peptide quality:

Warm up the peptide to room temperature prior to opening and weighing out portion of the peptide. The recommended time for warm up is 1 hour.
In a clean environment weigh out desired quantity of peptide quickly.
Store the remaining peptide in freezer, preferably below -200C within an enclosed case with dessicants.


How long can I store my peptide when it is in solution?


It is not recommended to keep excess peptides in solution. The shelf life of peptides in solution is very limited, especially for sequences containing cysteine, methionine, tryptophan, asparagine, glutamine, and N-terminal glutamic acid. In general, aliquot the necessary amounts of peptide for a few days and relyophilize remaining portions for long term storage if necessary.


How should I store my peptide when it is in solution?


If storage of peptides in solution is absolutely unavoidable, use sterile buffers at pH 5-6 and store aliquots at -200C to prolong the storage life of peptides in solution.


SOLUBILIZATION
How do I solubilize my peptide?


All solubility information is reported on the Certificate of Analysis received with your peptide. Please read the instructions carefully before starting. Solubility may very depending on the nature of the peptide. The most common dissolution process is 1 mg of peptide distilled in 1 ml of sterile water.


Why the solubility may vary from one peptide to the other?


Solubility is often one of the major challenges for researchers who work with peptides. Each amino acid exhibits its own physical characteristics. Amino acids such as leucine, isoleucine, and valine are very hydrophobic. Other amino acids like lysine, histidine, and arginine are hydrophilic. Thus, different peptides will have different solubilities, based on the content of the peptides.


What should I do if my peptide does not dissolve well?


As a general rule, peptides should first be dissolved in distilled, preferably sterile water. If solubility still remains a problem, try the following steps:
Sonication is helpful to dissolve the peptide
Small amounts of dilute(10%) aqueous acetic acid for basic peptides or aqueous ammonia for acidic peptides may help dissolution of these peptides.
It is also recommended that the peptide be dissolved to the highest possible concentration, and then diluted with water or buffer to the working concentration.(Note: The buffer should be added only after the peptide is completely in solution form because salts may cause aggregation)

Do I need to solubilize my peptide under specific pH?


It will also be stated on your Certificate of Analysis if any specific PH level is requested.


"ACN" on some solubility description, what does it mean?


ACN stands for Acetonitrile. It may be used in assisting dissolution of some peptides.


MY PEPTIDE
What kind of analysis is included in the Peptide Products?


All peptides come with complete Certificate of Analysis. Mass Spec, HPLC and Amino Acid Analysis(as a standard for catalog peptides and optional for custom peptides)


How do I know what is the purity of my peptide?


The purity is documented in the Certificate of Analysis package with your peptide. Included in the Certificate of Analysis is also the HPLC chromatogram.


What is the AMU unit?


AMU stands for Atomic Mass Unit. It is a common unit measure for peptide.


What is the 2% when I have a 98% purity peptide?


2% consists of truncated or deletion sequence.


What is the other 20% if my peptide content is 80%?


Salt and water.


STORAGE
How should I store my lyophilized peptide when I receive it?


You should store your peptide in the freezer of -200C or below immediately upon arrival.


Do you have any recommendation on the storage procedures?


Peptides are delivered in lyophilized powder form and often hydroscopic. Absorption of water will decrease stability of the peptide and may reduce overall peptide content. For best results,please note:
Place in closed dry environment with dessicants.
Upon arrival, always store lyophilized peptide in a freezer at -200C for maximum stability
Avoid using a frost-free freezer. Changes in moisture and temperature may affect peptide stability.
Ambient temperature during shipping does not affect product life and efficacy.


I live abroad and my peptide was out for few days during shipping and customs. Is it still good for my research?


In the lyophilized form and properly packaged, peptides in general, can stay in room temperature without being damaged. Please make sure to store in the freezer upon receipt.

[LuvMuhRoids]

GHRP-6 Growth Hormone Release Peptide

[jbigdog69]

It should be administered exactly like IGF-1

	IGF-1

[jbigdog69]

Abstract:

OBJECTIVE Both spontaneous and stimulated GH secretion are reduced in patients with hypothyroidism. The mechanisms involved in these alterations are not yet fully understood. GHRP-6 is a synthetic hexapeptide that releases GH both in vivo and in vitro. Its mechanism of action is unknown, but there is evidence that this peptide acts as a functional somatostatin antagonist at pituitary level. The aim of this study was to evaluate the GH response to GHRP-6 in patients with primary hypothyroidism and in normal controls.

DESIGN Patients with hypothyroidism and normal controls were randomly submitted to 3 tests with GHRH (100 mug i.v.), GHRP-6 (1 mug/kg i.v.) and GHRH + GHRP-6, on separate days.

PATIENTS Eleven patients with primary hypothyroidism were compared with 10 control subjects.

MEASUREMENTS GH, TSH and free T4 were measured by immunofluorometric assay and IGF-I by radioimmunoassay.

RESULTS Hypothyroid patients had markedly lower peak GH values (mean ± SE mug/l) after GHRH administration (4.1 ± 0.9) compared to control subjects (24.9 ± 5.1). After GHRP-6 injection hypothyroid patients had a significantly higher GH release (12.6 ± 1.9) than that obtained with GHRH, while in control subjects GH values were similar (22.1 ± 3.6). No significant differences in peak GH responses were observed following the administration of either GHRP-6 alone (controls 22.1 ± 3.6; patients 12.6 ± 1.9) or in combination with GHRH (controls 77.4 ± 15.0; patients 52.8 ± 10.9), despite the trend to smaller responses in hypothyroid patients.

CONCLUSION We have shown that patients with primary hypothyroidism have higher GH responses to GHRP-6 than to GHRH, which are markedly blunted. When GHRP-6 was associated with GHRH, a significant increase in the GH response was observed in these patients, which could suggest a role for somatostatin in this process. Our data suggest that thyroid hormones modulate GH release induced by GHRH and GHRP-6 through different mechanisms. However, additional studies are necessary to further elucidate this hypothesis.

	IGF-1

[LuvMuhRoids]

That's what I was told by another user. The only other information I gather from the above, which I got off the manufacture's website, is to add 1ml of sterile solution to every 1mg of peptide.

Originally Posted by jbigdog69

It should be administered exactly like IGF-1

[jbigdog69]

Originally Posted by LuvMuhRoids

That's what I was told by another user. The only other information I gather from the above, which I got off the manufacture's website, is to add 1ml of sterile solution to every 1mg of peptide.

Yea..I am not exactly sure the dosage as there are a lot of "unknown human info" about this particular peptide. However, it is just another way to stimulte HGH production inducing IGF-1 bennefits. So, IMO...I will stick with a long cycle of HGH with known bennefits and or a short 45 day cycle of IGF-1. Peace

	IGF-1

	Hgh 177-191

[LuvMuhRoids]

I been injecting it at 25ius which would be 250mcgs. Im an idiot. See there is no info on this stuff it's hard to figure out what to do. No wonder it hit me so hard. It is supposed to be 100mcgs per 10 slashes. So Im going to change up and do 25hcgs in the mornings instead.

I been on 3 days now and it hit me so hard it knocked me out like a drug. I got real sleepy after injection. What I found out is it lowers blood sugar. Up until today I had a huge protein/carb drink right after injection and I felt much better.

Also my appetite is immense. By the second day after I eat I feel like I need to eat again and my stomach rumbles no sooner after I ate. As for its growth hormone inducement I cant tell you if it is doing that. I need blood work for that.

	protein

[LuvMuhRoids]

Today is the 4th day on GHRP and I injected 25mcg's with my carb/protein shake for breakfast. Didnt feel sleepy at this time again.

	protein

[jbigdog69]

Damn Bro...Ur a sik mofo...but thats why we love ya...keep an eye on your bf% and vascularity for us if ya can. This is the area I think you will notice a pretty quick difference. peace