Abstract
The control on use of anabolic agents in meat producing animals is generally based on excreta or hair analysis. This strategy is not relevant to imported carcasses or retail meat. A single sensitive method for a wide range of anabolic steroids was developed. After extraction of the freeze-dried tissue, phase II metabolites were deconjugated, and extracted on a SPE polymeric stationary phase prior to saponification of ester residues. Liquid-liquid partitioning was used to separate the analytes into phenol containing molecules and 4-3-one compounds. SPE on silica and aminopropyle columns was performed before applying a specific derivatisation for each compound sub-group. GC-quadrupole MS permitted the detection of 23 steroids in the 5-100 ppt range. GC-high resolution MS in the selected ion monitoring mode (HR-SIM) was used as confirmatory technique.
Introduction
Although the utilisation of steroids is permitted and widely used in various countries in the world, their use as growth promoters in meat producing animals is prohibited in the European Union. Development of efficient analytical methods appropriate to tissue samples is necessary for checking importation into the EU (Boarding Inspection Post) as well as European production (slaughterhouse or retail level). Survey of steroid residues in tissue requires suitable analytical strategy assuring minimal performance in term of
a and
b risks, with a balance between efficient purification technique, sensitive and specific measurement, and productiveness/cost. Today, combination of gas chromatography and mass spectrometry allows control laboratories to screen for growth promoter residues in complex biological matrices at the ppt range and to identify unambiguously these ultra traces. The analytical procedure presented herewith proposed the detection of 23 steroids in meat and liver on a quadrupolar mass spectrometer below 100 ppt, and their identification according to the 93/256/EC directive thanks to high resolution measurement.
http://www.euroresidue.nl/ER_IV/Cont...%20226-231.pdf 
04-26-2005, 01:39 PM
Detection And Identification Of Anabolic Steroid Residues In Tissue 
Linear Mode
